Intra Cytoplasmic Sperm Insertion (ICSI) is a procedure by which a single sperm is inserted with the aid of a micro needle into the cytoplasm of a mature oocyte. This procedure is recommended for severe cases of male factor infertility.

The ICSI procedure is conducted as a part of a cycle in which the ovaries of the female partner or egg donor are stimulated with hormones to increase the production of oocytes. Mature oocytes are removed in our office using mild sedation and an ultrasound guided needle. The oocytes are separated and their maturity is assessed in the laboratory. The mature oocytes are injected one by one using a glass holding pipette to secure the egg and a very small micro needle through which the sperm is inserted. The egg is subsequently placed in culture medium and fertilization is determined 12-18 hours later.

Technically, the ICSI technique is a precise and delicate procedure. It requires top quality equipment, well trained and skilled personnel, and attention to detail. In the hands of experienced personnel more than 70% of the oocytes are fertilized with ICSI. Ten percent or less of the injected oocytes are lost in the process of injection, and the pregnancy rates are as good as with standard IVF. As more research is conducted on this technique if is projected that the results are going to improve the outcome of infertility therapy in the future.